Histologic study of bone-forming capacity on polydeoxyribonucleotide combined with demineralized dentin matrix
김석곤, 허창규, 이재훈, 김경욱, 김문영,
소속 상세정보
김석곤 ( Kim Seok-Kon ) - Dankook University College of Medicine Department of Anesthesiology and Pain Medicine
허창규 ( Huh Chang-Kyu ) - Dankook University College of Dentistry Department of Oral and Maxillofacial Surgery
이재훈 ( Lee Jae-Hoon ) - Dankook University College of Dentistry Department of Oral and Maxillofacial Surgery
김경욱 ( Kim Kyung-Wook ) - Dankook University College of Dentistry Department of Oral and Maxillofacial Surgery
김문영 ( Kim Moon-Young ) - Dankook University College of Dentistry Department of Oral and Maxillofacial Surgery
Abstract
Background: This study examined the osteoinductive activity of demineralized dentin matrix (DDM) from human and polydeoxyribonucleotide (PDRN) for nude mice.
Methods: Twenty healthy nude mice, weighing about 15~20 g, were used for the study. DDM from human and PDRN were prepared and implanted subcutaneously into the dorsal portion of the nude mice. The nude mice were sacrificed at 1, 2, and 4 weeks after grafting and evaluated histologically by hematoxylin-eosin and Masson’s trichrome staining. The specimens were also evaluated via a histomorphometric study.
Results: The DDM and PDRN induced new bone, osteoblasts, and fibroblasts in soft tissues. The histological findings showed bone-forming cells like osteoblasts and fibroblasts at 1, 2, and 4 weeks. New bone formation was observed in the histomorphometric study. In particular, the ratio of new bone formation was the highest at 2 weeks compared with the first week and fourth week.
Conclusions: In this study, we showed that the PDRN used in this experimental model was able to induce bone regeneration when combined to the DDM.
키워드
Polydeoxyribonucleotide (PDRN); Demineralized dentin matrix (DDM); Osteoinduction; Nude mice
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